Figure 6.

Metabolome profile of hypoxic preconditioned mesenchymal stem cells/multipotent stromal cells (MSCs). Following the experimental design schematized in Figure 2A, MSCs were cultured for 48 hours in standard culture media in either 20% or 1% O₂ (A and B, respectively), or for an additional 3 days under serum deprivation and 1% O₂ (C and D). Then, samples were processed and total metabolites determined by mass spectrometry (see Materials and Methods). Cluster analysis (upper left corner) was performed with total metabolites detected, while heat map shows only metabolites with common names.