Table 1.
Differentially expressed metabolites in hypoxic preconditioned mesenchymal stem cells/multipotent stromal cells (MSCs) as detected by mass spectrometry analysis
| BinBase name | Fold change | p value |
|---|---|---|
| A versus B (>1 higher in B) | ||
| 31547 | 0.7 | .002 |
| 42445 | 0.6 | .030 |
| Adenosine | 0.6 | .040 |
| Aspartic acid | 0.5 | .026 |
| Creatinine | 2.1 | .045 |
| Fumaric acid | 0.7 | .022 |
| Lactose | 1.4 | .014 |
| Malic acid | 0.7 | .050 |
| A versus C (>1 higher in C) | ||
| 21682 | 1.8 | .041 |
| 21811 | 2.3 | .015 |
| 31224 | 1.6 | .028 |
| Adenine | 0.5 | .016 |
| Aspartic acid | 0.6 | .037 |
| Beta-alanine | 0.5 | .018 |
| Citrulline | 1.9 | .029 |
| Creatinine | 0.5 | .012 |
| Glucose-6-phosphate | 0.6 | .026 |
| Guanosine | 0.5 | .034 |
| Isoleucine | 1.1 | .017 |
| Lactose | 1.7 | .036 |
| Leucine | 1.4 | .039 |
| Lysine | 1.6 | .042 |
| Phenylalanine | 1.4 | .040 |
| Serine | 1.4 | .012 |
| Threonic acid | 2.3 | .047 |
| Tyrosine | 1.4 | .014 |
| Uracil | 0.2 | .030 |
| Valine | 1.4 | .027 |
| C versus D (>1 higher in D) | ||
| 17068 | 0.8 | .007 |
| 34097 | 1.4 | .008 |
| Citrulline | 0.7 | .022 |
| Fumaric acid | 0.8 | .009 |
| Histidine | 1.1 | .012 |
| Lactose | 0.4 | .051 |
| Sorbitol | 0.7 | .008 |
| B versus D (>1 higher in D) | ||
| 47 | 1.1 | .034 |
| 257 | 1.4 | .049 |
| 657 | 1.2 | .016 |
| 1709 | 1.5 | .007 |
| 1875 | 1.2 | .013 |
| 1878 | 1.2 | .024 |
| 2810 | 0.7 | .011 |
| 18520 | 1.6 | .042 |
| 21664 | 1.2 | .020 |
| 21811 | 1.9 | .027 |
| 31222 | 2.6 | .002 |
| 100721 | 2.7 | .012 |
| 100768 | 1.4 | .050 |
| Beta-alanine | 0.5 | .010 |
| Creatinine | 0.3 | .036 |
| Glucose | 2.0 | .008 |
| Glycine | 1.2 | .008 |
| Glycyl proline | 2.3 | .042 |
| Guanine | 0.5 | .041 |
| Histidine | 1.4 | .018 |
| Inosine 5′-monophosphate | 2.1 | .049 |
| Lauric acid | 0.9 | .004 |
| Lysine | 1.6 | .004 |
| Nicotinamide | 0.7 | .006 |
| Serine | 1.3 | .047 |
| Trans-4-hydroxy-l-proline | 1.4 | .050 |
| Tyrosine | 1.4 | .025 |
| UDP-N-acetylglucosamine | 0.6 | .010 |
| Uracil | 0.2 | .037 |
Conditions (schematically described in Fig. 2A) are as follows: (A) MSCs cultured for 2 days in standard culture media in 20% O2; (B) MSCs cultured for 2 days in standard culture media in 1% O2; (C) MSCs cultured as in (A) and additional 3 days under hypoxia and serum deprivation; (D) MSCs cultured as in (B) and additional 3 days under hypoxia and serum deprivation. Only statistically significant differences are shown, where values of detected metabolites are expressed as ratios of one experimental condition over another.