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Confirmation of successful disruption of the C. jejuni fur gene by Southern hybridization. A probe consisting of an internal SspI fragment of C. jejuni fur (Fig. 1) was hybridized to HindIII-digested genomic DNA from C. jejuni NCTC 11168 (wild type; lane A) and AV17 (fur mutant; lane B). Marker sizes (in kilobases) are indicated on the right.
