FIG. 3.

Immunoblot analysis of separately expressed malK fragments. Cells of host strain ES25 harboring the described plasmids were grown in the presence of IPTG (except pSW7; for details, see Materials and Methods). At early stationary phase, the cells were harvested and subjected to SDS-PAGE. Subsequently, the proteins were transferred to nitrocellulose and probed with a MalK-specific polyclonal antiserum. The blot was developed with the enhanced chemiluminescence kit from Amersham. Lanes: 1, MalKN1 (pGS94-11); 2, MalKC1 (pGS94-8); 3, MalKN2 (pGS94-13); 4, MalKC2 (pGS94-14); 5, MalKN3 (pGS94-20); 6, MalKC3 (pGS95-6); 7, MalK (wild type) (pSW7).