Table 1.

Summary of size and particle number of EVs with sample volume used for EV isolation in different fish species 

Species and EV source	Size distribution by NTA or nano-flowcytometry (peaks) (nm)	Size distribution (peak) by DLS (nm)	Size distribution (peak) by TRPS (nm)	Particle number (particles/mL) by NTA	Particle number (particles/mL) by TRPS	Sample volume (μl)	Reference
Rainbow trout gut (large EV) RTgutGC supernatant		91–531 (164)	250–1250 (350)		6.7E + 10 ± 8.2E + 09	8,000	present data
Rainbow trout gut (small EV) RTgutGC supernatant		58–1106 (122.4)	75–225 (125)		1.3E + 11 ± 1.05E + 11
Rainbow trout gill (large EV) RTgill-W1 supernatant		21–255 (164)	250–1150 (350)		6.39E + 10 ± 3.1E + 10
Rainbow trout gill (small EV) RTgill-W1 supernatant		10–712 (164)	75–225 (125)		3.37E + 10 ± 52.26E + 10
Rainbow trout liver (large EV) RTL-W1 supernatant		58–2,000 (220)	250–1250 (350)		1.21E + 11 ± 4.89E + 10
Rainbow trout liver (small EV) RTL-W1 supernatant		68–458 (164)	75–225 (125)		1.68E + 11 ± 8.45E + 10
Zebrafish cell line liver (large EV) ZFL supernatant		68–2,000 (141.8)	250–1150 (350)		6.79E + 10 ± 3.09E + 10
Zebrafish cell line liver (small EV) ZFL supernatant		58–955 (190.1)	75–225 (125)		6.34E + 10 ± 4.37E + 10
Atlantic cod mucus	30–500 (142)			5.8e + 9		200	Magnadóttir et al., 2019
Atlantic cod mucus and plasma	30–400 (120)			6.5e + 8		200–250	Lange et al. 2019
Atlantic cod plasma	30–500 (160)			5.0–7.0e + 10		250	Magnadóttir et al. 2020
Atlantic salmon plasma	60–250 (124/106)			6.62e + 8—7.08e + 8		3,000	Muñoz et al., 2022
Salmon headkidney leukocytes supernatant	107.6–137			2.0–2.2E + 8		2,000	Smith et al. 2020
Coho salmon plasma	56–278 (190)			1.6E + 6–1.1E + 7		200	Leiva et al. 2021
Chinese tongue sole plasma	20–120 (95)			1.80e + 9—2.71e + 9		40,000	Sun et al. 2017
Chinese tongue sole plasma	100–400 (142)			2.40 ± 0.08e + 9		na	Zhao et al. 2021
Chinese tongue sole plasma	50–200*			5.28e + 8—2.71e + 9		na	Zhu et al. 2022