Figure 1.

Effect of CRAMP-34 on PAO1 pre-biofilm biomass and metabolic viability. (A) PAO1 biofilms were cultured for 72 hours at 37°C in 96-well plates. these biofilms were exposed to CRAMP-34 for 1 hour. Biomass quantification was conducted using crystal violet (CV). (B) After culturing PAO1 biofilms under similar conditions and post-exposure to CRAMP-34, the metabolic viability of the biofilm-associated bacteria was assessed using the formazan-based MTT assay. The significance of results was analyzed using an unpaired two-tailed t-test. Notably, *P < 0.05 and **P < 0.01 when compared to the control group.