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. 2023 Dec 13;13:1295311. doi: 10.3389/fcimb.2023.1295311

Figure 2.

Figure 2

CLSM imaging of CRAMP-34 treated PAO1 prebiofilms. PAO1 biofilms were formed for 72 h at 37°C on chambered coverglass slides. biofilms were treated with CRAMP-34 for 1 h at 37°C as described and subsequently stained with SYTO 9 and PI for 20 min in the dark. (A, B) The 3D and orthogonal views biofilm representation in the objective of 20X in the control group. (C, D) The 3D and orthogonal views biofilm representation of CRAMP-34 in the objective of 20X. (E) The total fluorescence intensity of biofilms. (F) represents the number of biofilms. (G) The bottom area of biofilms. (H) represents the volume of biofilms. (I) The total fluorescence intensity of viable or dead bacteria per unit biofilm area. (J) The total fluorescence intensity of viable or dead bacteria per unit biofilm volume. Unpaired t-test (two-tailed) was used to measure statistical significance. *P < 0.05, **P < 0.01 compared with the control group.