Extended Data Figure 4:

a. UMAP plot of scRNAseq analysis of F4/80⁺MHCII^hi/lo macrophages sorted from pancreas of Flt3-YFP mice treated with vehicle by 6 hourly i.p. injections every other day for one week. b. Quantification of Flt3-YFP⁺ and Flt3-YFP⁻ pancreas macrophages by cluster from UMAP in a, displayed as percentage of each cluster. c. UMAP plot of scRNAseq analysis of F4/80⁺MHCII^hi/lo macrophages sorted from pancreas of Flt3-YFP mice orthotopically implanted with the KP1 pancreatic cancer cell line. d. Quantification of Flt3-YFP⁺ and Flt3-YFP⁻ pancreas macrophages by cluster from UMAP in c, displayed as percentage of each cluster. e. UMAP plots displaying Timd4, Trem2, Folr2, H2-Aa, Cd74, and Cd14 expression in Csf1r and C1qa expressing pancreas macrophages from Flt3-YFP mice treated with cerulein by 6 hourly i.p. injections every other day for one week. f. Violin plots displaying expression of Lyve1 and Cx3cr1 for each cluster of Csf1r and C1qa expressing pancreas macrophages from Flt3-YFP mice treated with cerulein as in a. g. UMAP plots displaying Lyve1, Cx3cr1, and Ccr2 expression in F4/80⁺MHCII^hi/lo macrophages sorted from pancreas of Flt3-YFP mice treated as in a. h. UMAP plots displaying Lyve1, Cx3cr1, and Ccr2 expression in F4/80⁺MHCII^hi/lo macrophages sorted from pancreas of Flt3-YFP mice implanted with tumors as in c. i. Bar graph of normalized enrichment score (NES) values of gene sets upregulated in Flt3-YFP⁺ or Flt3-YFP⁻ pancreas macrophages within pancreatitis cluster 0 (from Fig. 3c). Data are presented as mean ± SEM unless otherwise indicated. n.s., not significant; *p <0.05. For comparisons between two groups, Student’s two-tailed t-test was used, except i where FDR was used.