TABLE 3.
Effects of different glcT mutations on the expression of the ptsGHI operona
Strain | Relevant glcT allele | β-Galactosidase activityb
|
|
---|---|---|---|
CSE | CSE + Glc | ||
QB5448 | Wild type | 15 | 490 |
GP103 | glcT1 | 895 | 420 |
GP116 | glcT-T102S D103T I109Q | 850 | 640 |
GP117 | glcT-T102S D103T | 1,030 | 675 |
GP118 | glcT-I109Q | 30 | 413 |
All strains contain a ΔLA ptsG′-′lacZ translational fusion at the amyE locus. The β-galactosidase specific activities were determined as described in footnote a of Table 2.
β-Galactosidase activity is expressed in units per milligram of protein.