TABLE 4.
Effects of different ptsG mutations on the expression of the ptsGHI operona
| Strain | Relevant genotype | β-Galactosidase activityb
|
|
|---|---|---|---|
| CSE | CSE + Glc | ||
| QB5448 | Wild type | 15 | 665 |
| GP101 | ptsG-H620D | 65 | 1,025 |
| GP122 | ptsG-H620A | 140 | 1,255 |
| GP102 | ptsG-C461D | 80 | 530 |
| GP121 | ptsG-C461A | 200 | 495 |
| GP111 | ptsG-ΔEIIB | 230 | 605 |
| GP113 | ptsG-ΔEIIBA | 360 | 720 |
a
All strains contain a ΔLA ptsG′-′lacZ translational fusion at the amyE locus. The β-galactosidase specific activities were determined as described in footnote a of Table 2.
b
β-Galactosidase activity is expressed in units per milligram of protein.