TABLE 1.
Antibodies for flow cytometry panels.
| Antigen | Clone | Fluorochrome | Supplier, catalog # |
|---|---|---|---|
| T cell panel | |||
| CD25 | PC61 | BV786 | BD Biosciences, 564023 |
| CD4 | RM4-5 | PerCP-e710 | eBioscience, 46-0042-80 |
| CD3 | 145-2C11 | FITC | BD Biosciences, 553062 |
| CD8 | 53–6.7 | APC-e780 | eBioscience, 47-0081-82 |
| FoxP3 | FJK-16s | APC | eBioscience, 17-5773-82 |
| Myeloid and B cell panel | |||
| CD45 | 30-F11 | BUV395 | BD Biosciences, 564279 |
| CD11b | M1/70 | e450 | eBioscience, 48-0012-82 |
| MHC II | M5/114.15.2 | BV650 | BD Biosciences, 563415 |
| CD11c | HL3 | BV786 | BD Biosciences, 563735 |
| Ly6G | 1A8 | FITC | BD Biosciences, 561105 |
| F4/80 | BM8 | PE | eBioscience, 12-4801-80 |
| Ly6C | AL-21 | PE-CF594 | BD Biosciences, 562728 |
| CD19 | MB19-1 | APC | eBioscience, 17-0191-81 |