TABLE 1.
Bacterial strains used in this study
| Strain | Genotype, relevant characteristics, and/or construction | Reference or source |
|---|---|---|
| E. coli MM294 | endE thiA hsdR17 supE44 | 3 |
| B. subtilis | ||
| PY79 | Prototrophic | 36 |
| PY1165 | spoIIE71 trpC2 | 4 |
| PY180 | spoIIE::Tn917ΩHU7 | 30 |
| PY184 | thyA thyB metB divIVB1 | P. Youngman |
| PMF16 | spoIIE-gfp; transformation of PY79 to Cmr with pBsIIE-mutGFP plasmid DNA | P. Fawcett |
| IB498 | divIVB1; transformation of PY79 to Cmr with pBGCDM3 plasmid DNA | This work |
| IB508 | divIVB1; transformation of PY79 to Kmr with pBGCDM3-kan plasmid DNA | This work |
| IB506 | spoIIE::Tn917ΩHU7 divIVB1; transformation of PY180 to Cmr with IB498 chromosomal DNA | This work |
| IB507 | spoIIE71 divIVB1; transformation of PY1165 to Cmr with IB498 chromosomal DNA | This work |
| IB509 | divIVB1 spoIIE-gfp; transformation of PMF16 to Kmr with IB508 chromosomal DNA | This work |
| IB515 | PY79 divIVB::cat (at KpnI); transformation of PY79 to Cmr with pBGCDdel plasmid DNA | This work |