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. 2024 Jan 1;14(2):608–621. doi: 10.7150/thno.89520

Figure 5.

Figure 5

Intramyocardial injection of human extracellular vesicles (EVs) reduce fibrosis and cardiomyocyte size in a model of rat sterile pericarditis. (A) Effect of pericarditis and EV treatment on hydroxyproline content (n=5-10) and Masson's Trichrome fibrosis (n=3-7). (B) Effect of pericarditis and EV treatment on atrial mass normalized to body weight (n=5-15). (C) Effect of pericarditis and intramyocardial injection of 109 EVs on cardiomyocyte size quantified using histological analysis of plasma membrane border (n=4 biological replicates with 5 technical replicates) after staining with wheat germ agglutinin (WGA) and 4′,6-diamidino-2-phenylindole (DAPI). Scale bar 20 μm. (D) Effect of pericarditis and intramyocardial injection of 109 EVs on the nucleus to cytoplasm ratio quantified using histological analysis (n= 5 random fields with 10 cells per field) after staining with WGA and DAPI. Scale bar 20 μm. One-way ANOVA with individual-mean comparisons by Šídák's multiple comparisons test. *P<0.05 vs. talc + vehicle. #P<0.05 vs. sham. EV, extracellular vesicles; veh, vehicle.