Table 2.
Selected polymer-based vehicles for mRNA delivery
| Category | Polymers | mRNA delivered | In vitro models | Route of administration | In vivo models | Category |
|---|---|---|---|---|---|---|
| Polyethyleneimine (PEI) | PEI2k-stearic acid (PSA) | HIV-1 gag | DC2.4 | s.c. | BALB/c mice | Polyethyleneimine (PEI) |
| CD-PEI | HIV gp120, OVA, Luc | DC2.4, MDCK, Calu-3 | intranasal administration | BALB/c mice | 55, 56 | |
| PEI-g-PEG | Luc | PC3, DC2.4 | i.v. | BALB/c mice | 57 | |
| Polyester | PACE | FLuc | Expi293F | i.v. | BALB/c mice | Polyester |
| PBAE-co-PCL | FLuc | Hela | i.v. | C57BL/6 mice | 62 | |
| PBAE-PGA-Ab | eGFP | K562-CD19, Jurkat-E6 | i.v. | NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice | 63 | |
| Dendrimers | PAMAM | Luc, cOVA | BHK21 | intranasal administration | C57BL/6 and BALB/c mice | Dendrimers |
| IAJD | Luc | HEK 293T | retro-orbital sinus injection | BALB/c mice | 65 | |
| Others | Chitosan coated PLGA | Z3 nec-mRNA | A549, MLE12 | i.p. | BALB/c mice, transgenic SP-B mice | Others |
| Oligoalkylamines-g-PAA8k | FLuc | A549, HepG2, NIH 3T3 | i.v. | BALB/c mice | 67 | |
| P(Asp-AED-ICA)-PEG | GFP | HEK 293, RAW 264.7 | / | / | 68 |
Note: i.v., intravenous; i.m., intramuscular injection; s.c., subcutaneous injection; i.p., intraperitoneal injection.