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. 2024 Jan 1;14(2):480–495. doi: 10.7150/thno.89592

Figure 2.

Figure 2

Regulation of M1 cortical activity by 5Cb Purkinje cells via thalamus. (A) Schematic representation of the virus injection sites in M1, Po and 5Cb of Pcp2/L7-cre mice. (B) Viral injection sites for GCaMP in M1, and eGFP in Po and 5Cb. (C) Averaged CaMKIIα+ neuron activity upon activation of Purkinje cells. Translucent red area indicates M1 CaMKIIα+ neuron activity in mice treated with saline (left, ChR2+Saline); translucent blue area indicates M1 CaMKIIα+ neuron activity in mice treated with CNO (right, ChR2+CNO). (D) Averaged ΔF/F values for ChR2+Saline and ChR2+CNO mice. (E) Schematic representation of the virus injection sites in M1, PF and 5Cb of Pcp2/L7-cre mice. (F-G) Viral injection sites for GCaMP in M1, and eGFP in PF and 5Cb. (H) Averaged CaMKIIα+ neuron activity upon activation of Purkinje cells. Translucent red area indicates M1 CaMKIIα+ neuron activity in ChR2+Saline mice; translucent blue area indicates M1 CaMKIIα+ neuron activity in ChR2+CNO mice. (I) Averaged ΔF/F values for ChR2+Saline and ChR2+CNO mice. Data are means ± SEM (n=5). M1: primary motor cortex; Po: posterior thalamic nuclear group; PF: parafascicular thalamic nucleus; 5Cb: the 5th lobule of the cerebellar vermis; CNO: Clozapine-N-oxide.