Figure 1.

The Process of Establishing Brain Tropic Cells. Establishing brain-tropic cells (brain metastasis cells) requires in vivo and in vitro screening. The selection process involves modifying cancer cells with reporter genes such as luciferase or GFP, which allows changes to be easily visualized, assessed, and prepared, using IVIS or MRI. Additional rounds of selection are then carried out. The modified cells are then reintroduced into the mice, usually after a period of growth outside the body.