Figure 3.

TG mice myocardia exhibited impaired mitochondrial function, heightened oxidative stress, and increased apoptosis. (A) Electron micrographs of heart tissue illustrate the morphology of mitochondria. (B) ATP content of heart tissues (n = 6). (C) OCR of primary neonatal cardiomyocytes was measured (n = 4). (D) Representative images and quantitative analysis of DHE staining (n = 5). (E, F) Cardiac MDA and NADPH content were measured in NTG and TG mice (n = 5). (G) Summary of cardiac oxylipins in NTG and TG mice (n = 3). (H) A representative cytochrome c blot and analysis in mitochondrial and cytosolic fractions (n = 4). Loading controls were established using β-actin and Cox IV. (I) Mouse hearts were stained with TUNEL (green) and DAPI (blue) in order to detect apoptotic cells (n = 4). TUNEL positive nuclei are quantified. The data is presented as the mean value plus or minus the standard error of the mean. Statistical significance is denoted by *p < 0.05 and **p < 0.01.