Figure 3.
The effects of inhibition or overexpression of DLEU2 on cell viability, migration and invasion in both A549 and H1299 or Huaier-treated A549 and H1299 cells. (A and B) The qRT-PCR assay for DLEU2 expression in A549 and H1299 cells transfected with DLEU2 siRNA (siDLEU2-1, siDLEU2-2, siDLEU2-3) or control (siNC). (C and D) The qRT-PCR to detect the DLEU2 level in NSCLC cells transfected with pcDNA3.1-DLEU2 (DLEU2) or vector control (vector). (E, G, I and K) A549 and H1299 cells transfected with DLEU2 siRNA (siDLEU2) or control (siNC) for 48 h. Colony formation assay reflected cell viability (E). Wound healing assay reflected cell migration (G). Transwell assay reflected cell invasion (I). Western blotting detected the protein expression of E-cadherin, Vimentin, MMP9 and VEGF (K). (F, H, J and L) A549 and H1299 cells treated with 4 mg/mL Huaier and pcDNA3.1-DLEU2 (Huaier + DLEU2) or Huaier and vector control (Huaier + vector) for 48 h. Colony formation assay (F), wound healing assay (H), transwell assay (J) and western blot assay(L) were performed.