Table 3.
The Role of 3D Bioprinting in the Study of CRC
| 3D Printing Method | Bioinks | Cells | Functions | References |
|---|---|---|---|---|
| Custom-built electro-hydrodynamic jet (E-jet) | Collagen | Human colorectal cancer cell line (HCT116), human umbilical vein endothelial cells (HUVECs) and human embryo lung fibroblasts (HELFs) | Research on tumor biology and development of personalized cancer treatment | [76] |
| Extrusion-based Bioprinting | Alginate with covalently bound RGD and nanofibrillar cellulose | Colorectal cancer cells (Caco-2) | Chemotherapy drug screening | [85] |
| Extrusion-based Bioprinting | Gelatin methacryloyl (GelMA)-nanoclay hybrid hydrogels | Colorectal cancer cell line (SW480) and primary colorectal cancer cell (hCC001) | Explored the new mechanism of biomaterials inducing CSCs | [79] |
| Extrusion-based Bioprinting | Gelatin-alginate hydrogel | Colorectal cancer cell line (SW620, SW480, LS174T and CACO2) | Evaluation of in vitro drug and radiation reactions | [84] |
| Extrusion-based Bioprinting | Hyaluronic acid and gelatin | Colorectal cancer cell line (HT29) and patient derived CRC tumoroids | Emphasized the importance of the glycosignature in the 3D bioprinting models | [82] |
| Dual-nozzle printing | Gelatin-alginate hydrogel | SW480 cells, tumor-associated macrophage and endothelial cells | Chemotherapy drug screening | [86] |