Map of the nas region. The open boxes represent open reading frames that encode NasA, -B, -C, -D, -E, and -F. The locations of the promoters are shown by P, and arrows represent the directions of transcription. The nucleotide sequences of the nasD promoter region are shown beneath the operon diagram. Asterisks indicate transcription start sites determined by the primer extension analysis in Fig. 4. Nucleotides in boxes are the putative TnrA binding site.