Expression of nasC-lacZ and nasF-lacZ fusions. Cells were grown in 2× YT medium containing 1% glucose and 20 mM phosphate buffer (pH 7.0) with 0.2% KNO3 (A) or 10 mM KNO2 (B) under anaerobic conditions. Time zero indicates the end of exponential growth. Closed circles, LAB1848 (wild type, nasF-lacZ); closed triangles, LAB2844 (wild type, nasC-lacZ); closed squares, LAB2012 (ΔnasB nasF-lacZ); open circles, LAB2008 (ΔnasD nasF-lacZ).