TABLE 4.
Nitrogen sourcea | β-Galactosidase activity (Miller units)b
|
|||
---|---|---|---|---|
LAB2854 (wild type) | LAB2862 (ΔresDE) | LAB2863 (fnr) | LAB2911 (tnrA) | |
Glutamate | 14 | 13 | 20 | 0.4 |
Nitrate | 40 | 21 | 43 | 0.6 |
Nitrite | 88 | 29 | 84 | <0.1 |
Ammonium | 0.7 | 0.6 | 0.6 | 0.4 |
Nitrate plus ammonium | 1.2 | NEc | NE | NE |
Nitrite plus ammonium | 2.3 | NE | NE | NE |
Cells were grown aerobically in TSS minimal medium with 0.5% glucose containing the indicated nitrogen sources.
Maximum activities during growth are shown (averages of three or more experiments). The standard error was less than 25%.
NE, not examined.