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. 1992 Nov;100(3):1580–1583. doi: 10.1104/pp.100.3.1580

Purification and Characterization of Cytosolic 6-Phosphogluconate Dehydrogenase Isozymes from Maize 1

Julia Bailey-Serres 1, Minh T Nguyen 1
PMCID: PMC1075824  PMID: 16653162

Abstract

Cytosolic isozymes of 6-phosphogluconate dehydrogenase were purified from roots of maize (Zea mays L.). The final preparation contained two 55-kD proteins. Affinity-purified dehydrogenases from a maize line that is null for both cytosolic 6-phosphogluconate dehydrogenase isozymes (Pgd1-null, Pgd2-null) lacked the 55-kD proteins. The substrate kinetics of the purified enzyme were determined.

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Selected References

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