Figure 6.

Size exclusion chromatography profiles of wild-type γC-crystallin and Y46D γC-crystallin mutant γC-crystallin. Superimposed tracings of the size standard alone are shown in a purple dotted line. (A) Association of αA-crystallin with wild type γC-crystallin incubated at room temperature (black), 45 °C (blue), 50 °C (red), and 55 °C (green). (B) Association of αA-crystallin with Y46D mutant human γC-crystallin incubated at room temperature (black), 45 °C (green), and 50 °C (orange). The left and right axes represent mAU for the crystallins and size standards, respectively.