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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Curr Protoc. 2021 Sep;1(9):e236. doi: 10.1002/cpz1.236

Figure 7.

Figure 7

CHEF gel to verify the quality and integrity of BAC DNA preparation. Here, BAC DNA contains the human BRCA1 gene isolated by TAR cloning in yeast (Kononenko et al., 2014). Lane 1 is BRCA1/BAC DNA linearized by NotI. Lane 2 is GeneRuler 1 kb plus DNA ladder (Thermo Fisher Scientific, cat. no. SM1331). Lane 3 is Pulse Marker™ 0.1–200 kb (MilliporeSigma, cat. no. D2291). Lanes 4, 5, and 6 are 0.25, 0.5, and 1 μg of uncut BRCA1/BAC DNA loaded onto the gel, respectively. As seen, the purified BRCA1/BAC DNA is predominately presented as covalently closed circular (ccc) molecules. TAR, transformation-associated recombination.