| Component | Volume/amount | Final amount/concentration |
|---|---|---|
|
| ||
| 40% (wt/vol) acrylamide:bisacrylamide (19:1) solution | 400 ml | 16% |
| 10× TBE | 100 ml | 1× |
| Urea | 420.42 g | 7 M |
| ddH2O | To 1 liter | — |
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