ST2-deletion delays liver regeneration after PHx. WT and suppression of tumorigenicity 2 (ST2)−/− mice were subjected to PHx. (A, B) Ki67 immunostaining was performed in liver sections collected at 36 h after PHx (scale bar: 100 μm). Ki67-positive hepatocytes were quantified (n = 8–13/group). (C,D) PCNA levels in liver tissues at 36 h after PHx were measured by immunoblotting and the ratios of PCNA/β-actin were quantified (n = 3/group). (E,F) BrdU incorporation was performed in liver sections at 36 h after PHx (scale bar: 100 μm). BrdU positive hepatocytes were quantified (n = 3-4/group). (G) The ratios of liver/body weight were analyzed at 36 h and 168 h after PHx (n = 4–13/group). Two-tailed unpaired Student’s t test was performed in (B), (D), and (F). Two-way ANOVA was performed in (G)