FIGURE 7.

P70S6K is involved in serotonin mediated hepatocyte proliferation. (A,B) Immunoblotting of phospho-p70S6K/p70S6K was performed in WT and ST2^−/− livers at 0 h and 12 h after PHx. The ratios of p-p70S6K/β-actin were quantified (n = 3/group). (C,D) WT and ST2^−/− livers treated with saline or DOI were collected at 12 h after PHx. Immunoblotting of phospho-p70S6K/p70S6K was performed and the ratios of p-p70S6K/β-actin quantified (n = 3/group). (E,F) Primary murine hepatocytes were isolated and treated with DOI after serum starvation. Phospho-p70S6K was measured by immunoblotting, and the ratios of p-p70S6K/β-actin from three independent experiments were quantified. (G,H) Primary murine hepatocytes were treated with DOI or an p70S6K inhibitor (p70S6Ki). The levels of PCNA were measured by immunoblotting. The ratios of PCNA/β-actin from three independent experiments were quantified. Two-way ANOVA was performed in (B), (D), (F), and (H)