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Kinetics of methylene H4MPT dehydrogenation with NADP+ as catalyzed by NADP+-dependent methylene H4MPT dehydrogenase from M. extorquens AM1. (A and C) Plots of 1/v versus 1/[S], where velocity (v) is given as micromoles of substrate converted per minute. (B and D) Replots of 1/apparent (app.) Vmax versus 1/[S]. Assay conditions were as follows: temperature, 30°C; pH 6.0; NADP+ and methylene H4MPT concentrations as indicated; 50 ng of purified methylene H4MPT dehydrogenase.
