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The Plackett–Burman design.
| Variables | Experiment levels | |
|---|---|---|
| −1 | +1 | |
| A: Corn starch (%) | 0.3 | 0.7 |
| B: Corn syrup powder (%) | 0.5 | 1.5 |
| C: MgSO4 (%) | 0.04 | 0.06 |
| D: MnSO4 (%) | 0.04 | 0.06 |
| E: Agitation speed (rpm) | 170 | 190 |
| F: Fermentation temperature (°C) | 34 | 38 |
| G: Airflow rate (vvm) | 1:1 | 1:1.4 |
| H: Inoculation volume (%) | 6 | 10 |
| I: Initial pH | 7.0 | 9.0 |
