TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| L. pneumophila | ||
| JR32 | Homogeneous salt-sensitive isolate of AM511 (Philadelphia-1 Smr r−m+) | H. Shuman (36) |
| PB102 | JR32 katB+katB::lacZ (katB fusion strain) | This work |
| PB117 | JR32 katB::ΩCm (katB null mutant) | This work |
| E. coli | ||
| UM383 | HB101 katG17::Tn10(Tet)katE::Tn10(Kan) | P. Loewen |
| UM383(pMMB207αB::katB) | UM383 with a PCR-amplified katB gene in the Hind/Pst site of pMMB207αB | This work |
| Plasmids | ||
| pJBZ280 | lacZ with polylinker upstream for constructing translational fusions; ColE1 ori (Kanr) | M. R. K. Alley (3) |
| pNPTS138 | Derivative of pLITMUS 38 cloning vector with nptI, RK2 oriT, and Bacillus subtilis sacB; Kanr | 27 |
| pHP45 ΩCm | pBR322 derivative containing the Cmr gene with transcriptional and translational terminators and a polylinker at each end of the antibiotic cassette (Ampr) | 28 |
| pMMB207αB | RSF1010 derivative; IncQ lacIq Genr Ptac oriT; multiple cloning site α complementation | 36 |
| pMMB207αB::katB | pMMB207αB with Hind/Pst fragment containing PCR-amplified katB gene in Hind/Pst site | This work |
| pNPTS138 katB::ΩCm | pNPTS138 with katB null allele (katB::ΩCm) in BamHI site | This work |