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. 2023 Dec 11;14:1290971. doi: 10.3389/fendo.2023.1290971

Figure 1.

Figure 1

Effects of food supplementation (FS) administration on body parameters. (A) Representative image of CTRL, HFD and FS (HFD +FS) male mice. (B–D) Body weight (B), body length (C) and abdominal circumference (D) in CTRL (n=12), HFD (n=12) and FS (n=12) mice. All data are reported as mean ± SEM. Different letters indicate statistical significance (p<0.05 or p<0.01). (E) Hematoxylin and eosin (H&E) staining of Bouin’s fixed CTRL, HFD and FS liver and testis sections (7 μm). Fat vacuole accumulation in hepatic cell was indicated by white arrowheads while in testis, detached germ cells were indicated by black arrowheads. Scale bar: 50 μm. (F) Immunofluorescence analysis of 4-Hydroxy-2-nonenal (4HNE) in cauda SPZ of CTRL, HFD and FS mice. White arrowheads and white asterisk represent 4HNE localization (green) in sperm head and tail, respectively. Nuclei were labeled with DAPI (blue). Scale bar: 5 µm.