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. 2023 Dec 11;14:1290971. doi: 10.3389/fendo.2023.1290971

Figure 2.

Figure 2

Effects of food supplementation (FS) administration on circRNA and FUS expression in SPZ. (A) Expression analysis of 9 circRNAs up-regulated in CTRL, HFD and FS sperm cells. qRT-PCR data are normalized using Cyclophilin expressed as fold expression (nfe) and reported as mean value ± S.E.M. a vs b: p< 0.01. (B) Immunofluorescence analysis of FUS protein in caput and cauda SPZ of CTRL, HFD and FS mice. White arrowheads and white asterisk represent FUS localization (red) in sperm head and tail, respectively. Nuclei were labeled with DAPI (blue). Scale bar: 5 µm. (C) Western blot analysis of FUS protein in caput and cauda SPZ of CTRL, HFD and FS mice (n=6 different samples for each experimental group in triplicate). Signals were quantified by densitometry analysis and normalized to Ponceau Red (Ponceau R). Data were expressed in OD values and reported as mean ± SEM. Different letters indicate statistical significance (p<0.05 or p<0.01).