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In vitro osteogenic differentiation: (a) ALP staining; (b) Alizarin Red staining; (c, d) ALP activity of MC3T3-E1 cells co-cultured with different sample extracts for 7 and 14 days; (e) Semi-quantitative analysis of mineralization levels; (f ∼ h) The expression of RUNX-2, OCN, and OPN genes of MC3T3-E1 cells co-cultured with different samples extracts for 7 days.
