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Thermostability of FEN protein. Lanes: 1, 21-nt marker; 2, oligonucleotide FS (37 nt); 3, FS after incubation of FS plus FA with FEN; 5, FS after incubation of FS plus FA with unheated FEN protein; 7, FS after incubation of FS plus FA with FEN protein preheated to 90°C for 15 min; lanes 4, 6, and 8, same as lanes 3, 5, and 7 but with omission of oligonucleotide FA.
