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. 2023 Nov 30;27(1):108599. doi: 10.1016/j.isci.2023.108599

Figure 1.

Figure 1

Feeder-free generation of endocardial and endothelial populations from iPSCs

(A) Schematic of differentiation timeline.

(B) Flow cytometry plots assessing mesoderm generation on day 4 of their respective differentiation strategies.

(C) Quantification of CD13+/CD56+ mesoderm generation (n = 4, ∗p < 0.05, two-way ANOVA with Sidak’s multiple comparisons test, error bars ±SEM).

(D) Flow cytometry plots assessing endocardial/endothelial differentiation efficiency on day 14 (also see Figure S1).

(E) Timeline of CD31+ endocardial differentiation on days 10, 12, and 14 (n = 4, ∗p < 0.05 for WTC11, ##p < 0.01 for MSN02-4, two-way ANOVA with Dunnett’s multiple comparisons test, error bars ±SEM).

(F) Quantification of CD31+ endocardial efficiency on day 14 (n = 5, ∗p < 0.05, two-way ANOVA with Sidak’s multiple comparisons test, error bars ±SEM).