BMP10-derived endocardial cells are distinct from VEGF-derived endothelial cells
(A) RT-qPCR analysis of cardiac and endocardial gene expression on day 14 endocardial and endothelial cells (n = 4, ∗p < 0.05, ∗∗p < 0.01, two-way ANOVA, error bars ±SEM).
(B) Representative confocal microscopy of MSN02-4 endocardial and endothelial cells, stained for CD31 (GFP) and NKX2-5 (RFP). Scale bar, 50 μm.
(C) Representative confocal microscopy of MSN02-4 endocardial and endothelial cells, stained for NFATc1 (GFP) and CDH5 (RFP) (also see Figure S2). White arrowheads = diffuse NFATc1 in the cytoplasm of endothelial cells. Scale bar, 50 μm.