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EndMT of endocardial cells generates VIC and VEC populations
(A) Flow cytometry plots following EndMT (also see Figure S1).
(B) Quantification of CD31− VIC and CD31+ VEC populations following EndMT (n = 4, error bars ±SEM).
(C) RT-qPCR analysis of mesenchymal and endothelial gene expression on day 14 endocardial cells and day 20/21 VICs/VECs (n = 4, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, two-way ANOVA with Tukey’s multiple comparisons test, error bars ±SEM).
