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KPS production by the rkp mutants. The phenol-water-extracted material from bacteria was analyzed by PAGE. For KPS-specific analysis, the gel was silver stained without oxidation. KPS production of the mutants carrying insertions Tn5-618 and Tn5-608 was reported earlier (15). The six new mutants produce no high-molecular-weight (HMW) KPS, and the lower-molecular-weight (LMW) polysaccharides isolated from the mutants show altered banding. wt, wild type.
