FIG. 3.

Organization of the rkp-2 region. (A) The positions, phenotypes, and insertion numbers of the Tn5 mutations are shown above the physical map and in panel B. Fix, symbiotic phenotype; DOC, DOC sensitivity; 16-3, phage φ16-3 sensitivity. Mutants carrying Tn5-202, Tn5-207, or Tn5-238 were obtained by homologous recombination (see Materials and Methods). (B) KPS and LPS production of the insertion mutants. Phenol-water extracts were separated in two parallel PAGE gels, and the gels were stained either for KPS or for LPS as described in Materials and Methods. Arrows indicate the LPS bands that were altered in the mutants. Wild-type control gels [AK631 (wt)] are also shown. HMW, high molecular weight; LMW, low molecular weight.