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. 2005 Apr 11;33(7):2090–2098. doi: 10.1093/nar/gki342

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Identification of the region of hTR that interacts with Ku70/80 (A) Various truncations of hTR were generated and tested against FL-hTR for their ability to compete for the interaction with Ku70/80. The molar excess amount used for each unlabeled competitor compared with labeled FL-hTR is as follows: for hTR fragments 10–451, 3.5 and 7 times (lanes 3 and 4); for hTR-10–354, 4 and 8 times (lanes 5 and 6); and for hTR-10–159, 11 and 22 times (lanes 7 and 8). Lane 1, FL-hTR probe alone; and lane 2, FL-hTR probe and Ku70/80. (B) Two other hTR truncations between nucleotides 354–451 were generated and tested in the same competition assays. The amount of each unlabeled hTR fragment used as a competitor (10–354, 354–451 and 404–451) was 3 and 6 times in molar excess compared with labeled FL-hTR (lanes 3–8). Lane 1, FL-hTR probe alone; and lane 2, FL-hTR probe and Ku70/80.