Figure 4.

Outcomes of exposure to RVFV-exposed mosquitoes in individual lamb in high exposure (left) and low exposure (right) groups. Rectal temperatures were taken twice daily, and more frequent during 3 and 4 DPF (A, B). Temperatures over 40.5 C were considered a fever (dashed line). Blood samples were daily assessed for viremia development (C, D), limit of detection is shown with the dashed line. At day 0, 4 and 8 DPF anti-RVFV antibody development was assessed by ELISA (E, F). A 50% signal was probable positive and 40% or less was considered positive (dashed lines). Upon euthanasia, RVFV presence was determined in 10% spleen and liver homogenates (G, H). PCR limit of detection shown in dashed line. Animal 4621 and 280 succumbed to infection on 3 and 4 DPF, respectively.