Table 1.

Primers for PCR-generated deletions of ZFP100

^aThe forward primers are named according to the first five amino acids, the first intact zinc finger repeat (after the hyphen) and the first amino acid number (in brackets) of ZFP100 which are encoded by the respective translation product. These primers each contain a T7 RNA polymerase promoter followed by an in-frame start codon (bold underlined). Amino acids shown in brackets were altered with respect to the natural ZFP100 sequence so that the start codon could be followed by a G residue (27).

^bThe internal reverse primers are designated with respect to the last intact zinc finger repeat (preceding the hyphen), the last three amino acids preceding the stop codon (asterisk), and the last amino acid of ZFP100 encoded by the respective translation product (in brackets). In-frame stop codons were introduced with the primers (bold underlined). STOP+30 (−871) designates a primer complementary to a sequence downstream of the natural stop codon.