TABLE 1.
Different classes of Fix−, K-antigen-deficient mutants have distinctive phage sensitivity profilesa
| Parental or mutant strain or class | Genotype | Sensitivityb to:
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| φM1 | φM5 | φM6 | φM7 | φM9 | φM10 | φM11 | φM12 | φM12H1 | φM14 | φM19 | φ16-3 | ||
| Parental strain AK631 | Wild type | R | R | R | R | R | R | R | R | +++ | R | R | +++ |
| Mutants | |||||||||||||
| rkp-1 | rkpA | R | R | + | +++ | ++ | +++ | +++ | +++ | +++ | +++ | +++ | R |
| rkpI | R | R | + | +++ | ++ | +++ | +++ | +++ | +++ | +++ | +++ | R | |
| rkpJ | R | R | + | +++ | +++ | +++ | +++ | +++ | +++ | ++ | +++ | R | |
| Class I | rkp-085 | R | R | R | +++ | R | + | +++ | ++ | +++ | + | ++ | R |
| rkp-143 | R | R | R | +++ | R | + | +++ | ++ | ++ | + | +++ | R | |
| rkp-162 | R | R | R | +++ | R | + | +++ | ++ | +++ | + | +++ | R | |
| rkp-163 | R | R | R | +++ | + | + | +++ | ++ | +++ | + | ++ | R | |
| rkp-180 | R | R | R | +++ | R | + | +++ | +++ | +++ | + | +++ | R | |
| rkp-181 | R | R | R | +++ | R | + | +++ | ++ | +++ | + | ++ | R | |
| Class II | rkp-132 | R | R | R | +++ | + | R | ++ | ++ | +++ | R | ++ | R |
| rkp-133 | R | R | R | +++ | + | R | ++ | ++ | +++ | R | ++ | R | |
| rkp-135 | R | R | R | +++ | + | R | +++ | +++ | +++ | R | ++ | R | |
| rkp-161 | R | R | R | +++ | + | R | +++ | ++ | +++ | R | ++ | R | |
| rkp-172 | R | R | R | +++ | + | R | ++ | ++ | +++ | R | ++ | R | |
| Class III | rkp-205 | R | R | R | ++ | + | + | ++ | ++ | ++ | + | ++ | R |
| Class IV | rkp-063 | R | R | R | + | R | R | +++ | ++ | +++ | R | ++ | R |
AK631 is resistant to all phage except φ16-3 and φM12H1. Mutations in genes located in the rkp-1 region, rkpA674, rkpI634, and rkpJ671, rendered AK631 sensitive to almost all phages tested with the exception of φM1 and φM5. Mutants in groups I, II, III, and IV also showed increased sensitivities to most phages tested. The mutants from the rkp-1 region and from groups I, II, III, and IV all show distinctive profiles of phage sensitivity and resistance.
Symbols: R, strain resistant to phage; +++, phage forms clear spot on bacteria; ++, phage will form slightly turbid spot on bacteria; +, phage will form a very turbid spot on bacteria and forms plaques with a reduced efficiency.