Fig. 1. Fluoxetine suppresses mast cell function in vitro.

(A) BMMC pre-sensitized with dinitrophenyl (DNP)-specific IgE were cultured with fluoxetine, sertraline, fluvoxamine, paroxetine, citalopram or vehicles, for 24 hours. DNP-HSA was added for 16 hours and cytokine concentrations in culture supernatants were determined by ELISA. N=6/group (B) BMMCs were treated with fluoxetine for the indicated time before IgE XL and DNP-HSA was added to crosslink for 16 hours. IL-6 levels in culture supernatant were measured by ELISA. N=4–6/group (C) BMMCs were treated as in (A) with the indicated concentrations of fluoxetine, and IL-6 levels were measured by ELISA. N=6/group (D) BMMCs were treated as in (A) with the indicated concentrations of fluoxetine, and viability as measured by flow cytometry after staining with propidium iodide. N=6/group. (E) BMMCs were treated as in (A), except that IgE XL was performed for 2 hours prior to the addition of monensin for 5 hours, then analyzed by flow cytometry. N= 16–19/group. (F) BMMCs cultured as in (A) were activated with IgE XL for 4 hours and RNA was collected using Trizol. Cytokine mRNA levels were determined by RT-qPCR. N=9/group. Data in A-D are representative of three independent experiments done in biological triplicates or quadruplicates. In (E-F), data are pooled from at least three independent experiments done in biological triplicates. All p values were determined by 1-way ANOVA (Tukeys test). *p<.05; **p<.001; ****p<.0001.