Fig. 7.

Melanoma cells pulmonary metastasis can be alleviated by CY12-RP2. A BALB/c mice were administered intravenously with 5 × 10⁵ melanoma cells B16-F10-Luc and analyzed for lung metastasis for 3 weeks using bioluminescence imaging according to the schematic chart. B Statistical analysis of bioluminescence imaging. C BALB/c mice's body weight was determined at the indicated time. D Organs including heart, liver, spleen, lung, and kidney were imaged using bioluminescence imaging. E Quantification analysis of lung metastases fluorescence in BALB/c mice. F Representative pulmonary nodules images in a B16-F10 lung metastasis model. The power field scale bar, 500 μm, 250 μm and 100 μm. G Cytokine levels including IL-1β, IL-2, IL-10, TGF-β, TNF-α, and IFN-γ in lung metastases model plasma were determined by ELISA. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 versus control