Fig. 6.

In vitro effect of metformin and CR treatments on PI3K/AKT proliferative pathway: Representative immunoblot analysis of total and different phosphorylated forms of the serine/threonine kinase AKT in KRAS^G12D (A) and KRAS^G12D/LKB1^del(B) cell lines. C-F Bar graphs of the quantification analysis of the different active forms of AKT normalized on RAN, used as housekeeping. Proteins were extracted after 72 h of treatment with 2 mM of metformin (MET) and 50% caloric restriction (CR). Non-treated cells were used as control. One-way ANOVA was used for statistical analysis and no statistically significant results were highlighted. Error bars indicate SD