Table 1.
Fluorochrome-conjugate monoclonal antibodies used for flow cytometry analysis
| Antibody | Clonality | Clone | Company | Dilution |
|---|---|---|---|---|
| CD45 (FITC) | Monoclonal | 30-F11 | BioLegend | 1:100 |
| CD3 (APC) | Monoclonal | 17A02 | BioLegend | 1:40 |
| CD4 (PerCP/Cyanine5.5) | Monoclonal | RM4-4 | BioLegend | 1:50 |
| CD8 (PE/Cyanine7) | Monoclonal | YTS156.7.7 | BioLegend | 1:50 |
| CD27 (Pacific Blue) | Monoclonal | LG.3A10 | BioLegend | 1:100 |
| CD25 8 APC/Fire 750) | Monoclonal | 3C7 | BioLegend | 1:20 |
| CD11b (Brilliant Violet 650) | Monoclonal | M1/70 | BioLegend | 1:20 |
| Ly-6G (Brilliant Violet 785) | Monoclonal | 1A8 | BioLegend | 1:150 |
| Ly-6C (Brilliant Violet 570) | Monoclonal | HK1.4 | BioLegend | 1:20 |
All antibodies were used as described in Material and Methods