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. 2024 Jan 2;43:4. doi: 10.1186/s13046-023-02879-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Expression of genes in ABCB1 locus is upregulated in PR cells. A Graphic visualization of ABCB1 amplicon on the locus 7q21.12. B-D Gene expression of ABCB4, ADAM22, TP53TG1 and SRI measured by RT-qPCR in PR (red) relative to CTR cells (blue) for the 3 indicated cell models. Brown-Forsythe and Welch ANOVA with Dunnet’s T3 post hoc test was used E Relative DNA amount of GAPDH, ABCB1, ABCB4, ADAM22, TP53TG1 and SRI in Patu-T CTR (blue), GR (green) and PR (red) cells, measured by DNA-qPCR and calculated as fold change (2^−ΔΔCt compared to the parental). F Representative images of Patu-T CTR, GR and PR cell metaphases stained with DAPI. Note absence of ecDNA. Scale bar: 20 μm. G-H Gene expression of SRI (G) and ABCB1 (H) in Patu-T PR cells transfected with the indicated siRNA SMARTpools (50 nM) measured by RT-qPCR relative to siKINASEpool samples. I Cell growth of Patu-T PR cells 72 h post-treatment with the indicated siRNA SMARTpools and paclitaxel concentrations, relative to siKINASEpool control samples. Verapamil (Vera) is used as positive control for ABCB1 inhibition