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Chiral HPLC analysis of benzylsuccinate formed in vitro from toluene and fumarate. (A) Sample containing benzylsuccinate produced from toluene and fumarate by permeabilized strain T cells (see text for experimental conditions); (B) the sample coinjected with a (+)-benzylsuccinate standard (0.8 mM); (C) the sample coinjected with a (−)-benzylsuccinate standard (0.8 mM). The identity of the compound eluting at ∼21 min is unknown.
